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cell plasma membrane staining kit with dio  (Beyotime)


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    Beyotime cell plasma membrane staining kit with dio
    Cell Plasma Membrane Staining Kit With Dio, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell plasma membrane staining kit with dio/product/Beyotime
    Average 99 stars, based on 35 article reviews
    cell plasma membrane staining kit with dio - by Bioz Stars, 2026-05
    99/100 stars

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    cell plasma membrane staining kit with dio  (Beyotime)
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    Beyotime cell plasma membrane staining kit with dio
    Cell Plasma Membrane Staining Kit With Dio, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell plasma membrane staining kit with dio/product/Beyotime
    Average 99 stars, based on 1 article reviews
    cell plasma membrane staining kit with dio - by Bioz Stars, 2026-05
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    cell plasma membrane staining with dio  (Beyotime)
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    Macrophage pyroptosis is triggered by injured TECs via secreted mediators. A) Experimental design for co‐culture of mouse kidney proximal tubular epithelial cells (TKPTS) and bone marrow‐derived macrophages (BMDMs). TECs were subjected to hypoxia and lipopolysaccharide (LPS) treatment for 24 h, followed by a 12‐h recovery in a fresh medium. Conditioned medium (CM) from injured TECs (HRL CM) and control TECs (CTL CM) was separately collected to treat BMDMs for 6 h, followed by nigericin (10 µ m ) for 2 h. Figure created with BioRender.com. B) Representative immunoblotting and quantitative data of pro‐caspase‐1, cleaved caspase‐1 (p20), GSDMD, and GSDMD‐N in the BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). C) Immunofluorescence <t>staining</t> of propidium iodide (PI) uptake and <t>plasma</t> <t>membrane</t> integrity in BMDMs treated with HRL CM or CTL CM followed by nigericin. Cells were stained with PI (red) to detect membrane permeabilization and <t>DiO</t> (green) to label the plasma membrane. Nuclei were counterstained with Hoechst (blue). Bar = 20 µm. D) Representative PI staining images of BMDMs treated with HRL CM or CTL CM ± nigericin. Bar = 50 µm. E) Quantification of PI⁺ BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). F) Quantification of LDH release from BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). G) ELISA analysis of IL‐1β and IL‐18 in supernatants of BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistically significant differences were determined by one‐way ANOVA followed by Bonferroni's test.
    Cell Plasma Membrane Staining With Dio, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell plasma membrane staining with dio/product/Beyotime
    Average 99 stars, based on 1 article reviews
    cell plasma membrane staining with dio - by Bioz Stars, 2026-05
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    		  Buy from Supplier
    cell membrane green fluorescence staining kit  (Beyotime)
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    Macrophage pyroptosis is triggered by injured TECs via secreted mediators. A) Experimental design for co‐culture of mouse kidney proximal tubular epithelial cells (TKPTS) and bone marrow‐derived macrophages (BMDMs). TECs were subjected to hypoxia and lipopolysaccharide (LPS) treatment for 24 h, followed by a 12‐h recovery in a fresh medium. Conditioned medium (CM) from injured TECs (HRL CM) and control TECs (CTL CM) was separately collected to treat BMDMs for 6 h, followed by nigericin (10 µ m ) for 2 h. Figure created with BioRender.com. B) Representative immunoblotting and quantitative data of pro‐caspase‐1, cleaved caspase‐1 (p20), GSDMD, and GSDMD‐N in the BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). C) Immunofluorescence <t>staining</t> of propidium iodide (PI) uptake and <t>plasma</t> <t>membrane</t> integrity in BMDMs treated with HRL CM or CTL CM followed by nigericin. Cells were stained with PI (red) to detect membrane permeabilization and <t>DiO</t> (green) to label the plasma membrane. Nuclei were counterstained with Hoechst (blue). Bar = 20 µm. D) Representative PI staining images of BMDMs treated with HRL CM or CTL CM ± nigericin. Bar = 50 µm. E) Quantification of PI⁺ BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). F) Quantification of LDH release from BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). G) ELISA analysis of IL‐1β and IL‐18 in supernatants of BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistically significant differences were determined by one‐way ANOVA followed by Bonferroni's test.
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    Average 99 stars, based on 1 article reviews
    cell membrane green fluorescence staining kit - by Bioz Stars, 2026-05
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    cell plasma membrane staining kit  (Beyotime)
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    Beyotime cell plasma membrane staining kit
    Macrophage pyroptosis is triggered by injured TECs via secreted mediators. A) Experimental design for co‐culture of mouse kidney proximal tubular epithelial cells (TKPTS) and bone marrow‐derived macrophages (BMDMs). TECs were subjected to hypoxia and lipopolysaccharide (LPS) treatment for 24 h, followed by a 12‐h recovery in a fresh medium. Conditioned medium (CM) from injured TECs (HRL CM) and control TECs (CTL CM) was separately collected to treat BMDMs for 6 h, followed by nigericin (10 µ m ) for 2 h. Figure created with BioRender.com. B) Representative immunoblotting and quantitative data of pro‐caspase‐1, cleaved caspase‐1 (p20), GSDMD, and GSDMD‐N in the BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). C) Immunofluorescence <t>staining</t> of propidium iodide (PI) uptake and <t>plasma</t> <t>membrane</t> integrity in BMDMs treated with HRL CM or CTL CM followed by nigericin. Cells were stained with PI (red) to detect membrane permeabilization and <t>DiO</t> (green) to label the plasma membrane. Nuclei were counterstained with Hoechst (blue). Bar = 20 µm. D) Representative PI staining images of BMDMs treated with HRL CM or CTL CM ± nigericin. Bar = 50 µm. E) Quantification of PI⁺ BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). F) Quantification of LDH release from BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). G) ELISA analysis of IL‐1β and IL‐18 in supernatants of BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistically significant differences were determined by one‐way ANOVA followed by Bonferroni's test.
    Cell Plasma Membrane Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell plasma membrane staining kit/product/Beyotime
    Average 99 stars, based on 1 article reviews
    cell plasma membrane staining kit - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Macrophage pyroptosis is triggered by injured TECs via secreted mediators. A) Experimental design for co‐culture of mouse kidney proximal tubular epithelial cells (TKPTS) and bone marrow‐derived macrophages (BMDMs). TECs were subjected to hypoxia and lipopolysaccharide (LPS) treatment for 24 h, followed by a 12‐h recovery in a fresh medium. Conditioned medium (CM) from injured TECs (HRL CM) and control TECs (CTL CM) was separately collected to treat BMDMs for 6 h, followed by nigericin (10 µ m ) for 2 h. Figure created with BioRender.com. B) Representative immunoblotting and quantitative data of pro‐caspase‐1, cleaved caspase‐1 (p20), GSDMD, and GSDMD‐N in the BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). C) Immunofluorescence staining of propidium iodide (PI) uptake and plasma membrane integrity in BMDMs treated with HRL CM or CTL CM followed by nigericin. Cells were stained with PI (red) to detect membrane permeabilization and DiO (green) to label the plasma membrane. Nuclei were counterstained with Hoechst (blue). Bar = 20 µm. D) Representative PI staining images of BMDMs treated with HRL CM or CTL CM ± nigericin. Bar = 50 µm. E) Quantification of PI⁺ BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). F) Quantification of LDH release from BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). G) ELISA analysis of IL‐1β and IL‐18 in supernatants of BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistically significant differences were determined by one‐way ANOVA followed by Bonferroni's test.

    Journal: Advanced Science

    Article Title: Tubule‐Derived IFN‐α Promotes GSDMD‐Mediated Macrophage Pyroptosis to Drive Renal Inflammation and Fibrosis Through JAK2/STAT2 Activation

    doi: 10.1002/advs.202512278

    Figure Lengend Snippet: Macrophage pyroptosis is triggered by injured TECs via secreted mediators. A) Experimental design for co‐culture of mouse kidney proximal tubular epithelial cells (TKPTS) and bone marrow‐derived macrophages (BMDMs). TECs were subjected to hypoxia and lipopolysaccharide (LPS) treatment for 24 h, followed by a 12‐h recovery in a fresh medium. Conditioned medium (CM) from injured TECs (HRL CM) and control TECs (CTL CM) was separately collected to treat BMDMs for 6 h, followed by nigericin (10 µ m ) for 2 h. Figure created with BioRender.com. B) Representative immunoblotting and quantitative data of pro‐caspase‐1, cleaved caspase‐1 (p20), GSDMD, and GSDMD‐N in the BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). C) Immunofluorescence staining of propidium iodide (PI) uptake and plasma membrane integrity in BMDMs treated with HRL CM or CTL CM followed by nigericin. Cells were stained with PI (red) to detect membrane permeabilization and DiO (green) to label the plasma membrane. Nuclei were counterstained with Hoechst (blue). Bar = 20 µm. D) Representative PI staining images of BMDMs treated with HRL CM or CTL CM ± nigericin. Bar = 50 µm. E) Quantification of PI⁺ BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). F) Quantification of LDH release from BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). G) ELISA analysis of IL‐1β and IL‐18 in supernatants of BMDMs treated with HRL CM or CTL CM ± nigericin ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistically significant differences were determined by one‐way ANOVA followed by Bonferroni's test.

    Article Snippet: PI staining and Cell Plasma Membrane Staining with DiO (Green Fluorescence) were performed using the Beyotime kit (C1734, C1993) as per the protocol.

    Techniques: Co-Culture Assay, Derivative Assay, Control, Western Blot, Immunofluorescence, Staining, Clinical Proteomics, Membrane, Enzyme-linked Immunosorbent Assay